ABSTRACT
Network pharmacology method was adopted in this study to explore the active compounds and mechanism of Tongsaimai tablets for atherosclerosis. In molecular docking and molecular-target protein network analysis, 97 molecules in Tongsaimai tablets showed good interaction with the atherosclerosis-related target protein (docking score ≥ 7), and 37 molecules of them could act on more than 2 targets (≥ 2) with higher betweenness, suggesting that these 37 molecules might be the main active compounds group in Tongsaimai tablets for atherosclerosis treatment. Furthermore, the predicted active compounds contained more flavonoids and saponins, reminding more attention should be paid on flavonoids and saponins in study of effective compounds and quality standards of Tongsaimai tablets. Targets network analysis showed that, the active compounds of Tongsaimai tablets could regulate inflammation, stabilize plaque, protect vascular endothelial cell, regulate blood lipid and inhibit blood coagulation through acting on the main 22 target proteins, such as Toll-like receptors (TLR1, TLR2), matrix metalloproteinase (MMP1, MMP2, MMP3, MMP9), angiotensin converting enzyme (ACE), leukotriene A4 hydrolase (LTA4-H), 5-lipoxidase (5-LOX), peroxisome proliferators-activated receptors (PPARα, PPARγ). These active compounds can participate in regulating different pathologic stages of atherosclerosis and thus treat atherosclerosis finally. This study revealed the main active compounds and possible mechanism of Tongsaimai tablets for treatment of atherosclerosis and meanwhile, verified the characteristics of multi-components, multi-targets and integral regulation for Tongsaimai tablets, providing theoretical references for the following systematic laboratory experiments on effective compounds and action mechanism of Tongsaimai Tablet.
ABSTRACT
Objective: To compare the difference of therapeutical effect with Tongsaimai Tablet, Tongxinluo Capsula, Buchang Naoxintong Capsula, Fufang Xueshuantong Capsula and Xuesaitong Capsula on focal cerebral ischemia of rats. Methods: The focal cerebral ischemia of rats was made by electric coagulation with middle cerebral artery (MCA). The score of ethology, the area of cerbral infarction, AngI, AngII in plasma, C-reactive protein (CRP) in serum, and NF-κB expression were examined to observe the effect of these five Chinese patent medicines by ig administration of Tongsaimai Tablet (0.239 g/kg), Tongxinluo Capsula (0.281 g/kg), Buchang Naoxintong Capsula (0.432 g/kg), Fufang Xueshuantong Capsula (0.405 g/kg), and Xuesaitong Capsula (0.027 g/kg) twice a day for 3 d. Results: Every group of the five Chinese patent medicines could significantly reduce the area of cerebral infarction and the expression of NF-κB. The score of ethology was more lower with Xuesaitong Capsula. The content of IL-6 in plasma was cut down greatly by Tongxinluo Capsula, Buchang Naoxintong Capsula, Xuesaitong Capsula, and Tongsaimai Tablet. The content of CRP in serum was reduced with Xuesaitong Capsula and Buchang Naoxintong Capsula. The content of AngII in plasma was degraded by Tongsaimai Tablet (P<0.05, 0.01). Conclusion: Every Chinese patent medicine can inhibit the release of inflammatory factor at varying degrees. These five Chinese patent medicines can degrade the expression of NF-κB so as to ameliorate cerebral ischemia, and Tongsaimai Tablet can cut down the content of AngII in plasma to lessen the symptom of cerebral ischemia.
ABSTRACT
AIM:To investigate the effect of TSM tablet on rat' s atherosclerotic model' s endothelial cell and explore the mechanism of it.METHODS:Fifty Wistar rats were randomly assigned into five groups:control group,model group,Simvastatin group,TSM high group,TSM low group.All groups were fed with high fat diet and vitamin D_3 except for control group to set up atherosclerosis model.After 12 weeks we detected circle endothelial cell,angiotensin Ⅱ and electron microscopy morphology of arteries.RESULTS:Level of circulating enthelia cell(CEC)and Ang Ⅱ in model rats were significantly higher.TSM can reduce level of the CEC and Ang Ⅱ.Model group rat' s artery endothelial cells were severely damaged under electron microscopy;rat's artery endothelial cells in TSM group were basically intact and its internal elastic membrane was unbroken on it thickness was even,without significant lesion.CONCLUSION:TSM by reducing the number of CEC,the level of Ang Ⅱ used for experimental atherosclerotic endothelial cell in rats has the protective effect.
ABSTRACT
AIM:To investigate the effect of TSM tablet on rat's atherosclerotic model's endothelial cell and explore the mechanism of it.METHODS:Fifty Wistar rats were randomly assigned into five groups:control group,model group,Simvastatin group,TSM high group,TSM low group.All groups were fed with high fat diet and vitamin D3 except for control group to set up atherosclerosis model.After 12 weeks we detected circle endothelial cell,angiotensinⅡ and electron microscopy morphology of arteries.RESULTS:Level of circulating enthelia cell(CEC) and AngⅡin model rats were significantly higher.TSM can reduce level of the CEC and AngⅡ.Model group rat's artery endothelial cells were severely damaged under electron microscopy;rat's artery endothelial cellsin TSM group were basically intact and its internal elastic membrane was unbroken on it thickness was even,without significant lesion.CONCLUSION:TSM by reducing the number of CEC,the level of AngⅡ used for experimental atherosclerotic endothelial cell in rats has the protective effect.